The seeds of elite genotypes (CSMCRI-JC-1, CSMCRI-JC-2 and
CSMCRI-JC-3) of J. curcas were obtained from Central Salt & Marine
Chemicals Research Institute (CSMCRI) experimental plantation on
wastelands at Chorvadla, India (21◦75N, 72◦14E) for the present
study. Seedcoats were removed and then surface sterilized with
0.1% mercuric chloride (HgCl2) for 15 min and rinsed five times
in sterile distilled water.