A sub-sample (1 g) of each sample was transferred into a
universal bottle containing 9 mL of Ringer’s solution and
then serially diluted from 10-1 to 10-4 before 100 μL of each
suspension was plated onto one of eight types of isolation
media (10% strength): R2A[17], R2A with 0.4% (w/v) sodium
propionate (modified from [15]), R2A with
50 μg∙mL-1 rose Bengal (modified from [15]), Gauze’s medium
2, starch casein nitrate (SCN) agar[18], tryptic soy agar
(Difco laboratories), tryptic soy agar with 0.1% (w/v) starch
and tryptic soy agar with 0.1% (w/v) colloidal chitin.
Cycloheximide (25 μg∙mL-1) and nystatin (25 μg∙mL-1)
were added to all isolation media to suppress the growth of
fungi.
A sub-sample (1 g) of each sample was transferred into auniversal bottle containing 9 mL of Ringer’s solution andthen serially diluted from 10-1 to 10-4 before 100 μL of eachsuspension was plated onto one of eight types of isolationmedia (10% strength): R2A[17], R2A with 0.4% (w/v) sodiumpropionate (modified from [15]), R2A with50 μg∙mL-1 rose Bengal (modified from [15]), Gauze’s medium2, starch casein nitrate (SCN) agar[18], tryptic soy agar(Difco laboratories), tryptic soy agar with 0.1% (w/v) starchand tryptic soy agar with 0.1% (w/v) colloidal chitin.Cycloheximide (25 μg∙mL-1) and nystatin (25 μg∙mL-1)were added to all isolation media to suppress the growth offungi.
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