Fruit fingers withdrawn randomly from each treatment and three fingers were sealed in a 2.6 L plastic container and then held for 2 h at 25 °C. The fruit in each treatment were divided into three replicate groups to measure the ethylene production rate. One milliliter headspace gas was withdrawn from the container and then injected into a gas chromatograph (GC-2010; Shimadzu, Kyoto, Japan) equipped with a 30 m HP-PLOT Q capillary column (Agilent Technologies, USA) and a flame ionization detector (FID). Ethylene production rate was expressed on a fresh weight basis as μg kg−1 s−1.
Respiration rate was determined by the methods with a LI-6262 CO2/H2O Analyzer (LI-6262, LI COR, America) (Huang and Jiang, 2012). Fruit fingers withdrawn randomly from each treatment and then three fingers were sealed into a box that connects the LI-6262 CO2/H2O Analyzer before the amount of CO2 was recorded for 4 min. The fruit in each treatment were divided into three replicate groups to measure the respiration. Respiration rate was expressed on a fresh weight basis as rate of CO2 production on a fresh weight basis as mg kg−1 s−1.