The region of the b-tubulin monomer examined encodes the Nterminal
domain [that includes the first 205 residues in which parallel
b-strands (B) alternate with a-helices (H)] and helices H6 and H7
(residues 206–240) of the intermediate domain (Nogales et al., 1998).
Furthermore, a 550–600 bp-long region of the calmodulin gene
(cmd), flanking parts of the second and fifth exons, was amplified and
sequenced using the primers cmd–f [59-GATTCCCTCACCGAAGAGCA-
39; present study, a modification of the cmdD3 primer (Wang &
Zhuang, 2007) based on the cmd sequence from the genome of
Penicillium chrysogenum Wisconsin 54–1255 (van den Berg et al.,
2008; Supplementary Table S1, available in IJSEM Online] and cmdA1
(59-GCCTCACGGATCATCTCGTC-39; Wang & Zhuang, 2007), and
the fungal b-tubulin gene sequence available from GenBank (NCBI).