Microstructure
A scanning electron microscope (Model JSM-5310LV; JEOL Ltd.,
Tokyo, Japan) was used to examine the dried shiitake mushrooms’
microstructure. A 1 mm thick slice of the dried samples was prepared,
positioned on an SEM stub, then sputter-coated with gold
(JEOL JEC-1200 sputter coater). All samples were examined under
high vacuum conditions at an accelerating voltage of 20.0 kV.