To evaluate the potential for aerosol contamination during microcentrifugation,
capped and uncapped tubes of amplification
product were spun for one minute at full speed next to capped or
uncapped tubes of water (20 mL), genomic DNA (10 ng), or amplification
product (20 mL) of another genotype. We normally perform
a brief full speed pulse spin. Capped or uncapped tubes of water,
genomic DNA, or amplification product were placed on the
bench next to the microfuge. The effect of aerosol generated during
pipetting was evaluated by ejecting tips containing residual genomic
DNA or amplification product in front of open tubes of water
(20 mL) or genomic DNA (10 ng)