Similarly, in the present study, post-thaw motility, duration of
motility, and viability values increased in case of 1.5 mg adding
rate of CLC when carp sperm treated in the extender (Table 1).
The above reported results are in generally agreement with the
presented results in Table 1 related with cryosurvival rate. In addition,
there were some differences regarding amount of CLC
between mammalian and fish sperm and also treating of CLC to
obtain ideal cryosurvival rate. It is noted that sperm have various
degree of cold shock susceptibility depending on the different
membrane phospholipid composition as well as the membrane
cholesterol to phospholipid molar ratio [14]. It is reasonable to
assume that obtained different results between species is due to
their different phospholipid, protein or carbohydrate contents of
sperm membranes and different sperm head dimensions and
structures in the present study. Cholesterol and phospholipid rates
of mammalian and carp sperm are very significant in terms of
cryosurvival. It has been also determined that species with greater
cholesterol to phospholipid ratio such as rabbits and human have