By considering the lacunae, a series of in vitro toxicological studies were carried out to ascertain the safety and genotoxic profile of KalmColdTM, following scrutiny of its cytotoxicity profiles in the tester strains of S. typhimurium and CHO-K1 cells. The three in vitro models employed for screening the genotoxic potential of KalmColdTM were Ames test, CA and MN assays both in the presence and absence of metabolic activation.