2.6. Bio-barcode assay
A solution containing two tDNAs in one PCR tube was heated at 95 ◦C for 10 min to separate the dsDNA into ssDNA and then cooled rapidly at 4 ◦C.
Serially diluted DNA samples (40L) were mixed with 0.8mg MNP-2pDNA in 200L assay buffer (10mMPBS
buffer, 0.15M NaCl, 0.1% SDS, pH 7.4). The hybridization reaction
was maintained at a temperature of 45 ◦C for 1 h in an incubator.
After hybridization, the MNP-2pDNA/tDNA was washed twice with
the assay buffer, then resuspended in 200L assay buffer. Meanwhile,
the functionalized 1pDNA-AuNP-bDNA-NTs complex was
centrifuged at 13,000rpm for 20 min and the unreacted thiolated
oligonucleotides in the supernatant were removed. The purified
1pDNA-AuNP-bDNA-NTs complex was resuspended in 200L
assay buffer, and 40L was then added into 200L solution containing
the MNP-2pDNA/tDNA.
2.6. Bio-barcode assay A solution containing two tDNAs in one PCR tube was heated at 95 ◦C for 10 min to separate the dsDNA into ssDNA and then cooled rapidly at 4 ◦C. Serially diluted DNA samples (40L) were mixed with 0.8mg MNP-2pDNA in 200L assay buffer (10mMPBSbuffer, 0.15M NaCl, 0.1% SDS, pH 7.4). The hybridization reactionwas maintained at a temperature of 45 ◦C for 1 h in an incubator.After hybridization, the MNP-2pDNA/tDNA was washed twice withthe assay buffer, then resuspended in 200L assay buffer. Meanwhile,the functionalized 1pDNA-AuNP-bDNA-NTs complex wascentrifuged at 13,000rpm for 20 min and the unreacted thiolatedoligonucleotides in the supernatant were removed. The purified1pDNA-AuNP-bDNA-NTs complex was resuspended in 200Lassay buffer, and 40L was then added into 200L solution containingthe MNP-2pDNA/tDNA.
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