We applied the previously established treatment conditions with TLP to determine whether the shift in CD4+ T cell proliferation can be normalized in the absence of detectable virus replication in the cells previously exposed to HCV. Briefly, approximately 5 × 106 cells were incubated in duplicate with CHC-1 or CHC-2 plasma under conditions described above in the presence or absence of 4 μM TLP in 0.5 % DMSO.