DNA isolation
DNA was isolated using the protocol of Doyle and Doyle (1990) that
was determined to be suitable for DNA isolation of all tomato
cultivars including F1, F2 and BC1 populations. To extract DNA, a
middle size (3 by 5 cm) leaf was sampled from each plant and
placed in an Eppendorf tube with adding extraction buffer. The DNA
was recovered by eluting with 100 μl of sterile distilled water.