The most common nano-particle employed in this field is Na–MMT which significantly increased resistance of biopolymers
against water, improved mechanical properties and decreased permeability [11,12]. The current research focus on some aspects of using Na–MMT to reinforcing the biopolymer. The central composite design (CCD) at three levels combined with response surface methodology (RSM) has been applied in this investigation. The effects of Na–MMT and glycerol on transparency, hydrophobicity and reductionin UV transmittance of corn starch films were studied.