However, germination of SM101 and NB16 spores with AK at 40 C followed by ethanol (70%)
treatment resulted in reduction of 2.14 and 0.72 log CFU/ml,
respectively (Fig. 2A and B). While RT-germination led to a signifi-
cant (p < 0.05) killing of SM101 spores with ethanol, this effect was
not observed with NB16 spores, as they were unable to germinate
with AK at RT (Fig. 2B). Extending treatment time up to 15 min did