Experimental set-up
Three experiments were carried out to investigate
15
N/
14
N and
13
C/
12
C fractionation in the
Collembolan P. fimata. The first experiment investigated
changes due to variations in food quality
represented by an altered C/N ratio of the agar
used as culture media for the fungus A. gibberosa.
P. fimata was fed with A. gibberosa at four
different C/N levels. The second experiment
analysed the effects of starvation. The Collembola
were reared with C. globosum, then the fungus was
removed and animals were kept without food
supply for additional four weeks. Specimens were
sampled at day 0 (non-starved), 2, 4, 8, 14, 21 and
28. The third experiment investigated variations in.