Fig. 4, but the maximum concentration of total sugar determined by the phenol-sulfuric acid method is obviously higher than the sum of main sugars. Moreover, the concentrations at Day 120 were apparently different: 43 mg ml1 for total sugar by the phenol-sulfuric acid method, while negligible for sum of main sugars. Meanwhile, broad unknown peaks with very low height were observed in the elution positions for oligosaccharides on HPLC. These results indicate presence of oligosaccharides in the sap of the stored trunks since the phenol-sulfuric acid method counts oligosaccharides in
addition to mono- and di-saccharides which can be determined
by the HPLC conditions used in this study.