Retrograded amylotype corn starch was prepared according to
Zhao and Lin method (Zhao & Lin, 2009) (Fig. 1). High-amylose corn
starch (10 g) was mixed with 40 mL of distilled water (starch:water,
1:4), and the mixture was then pressure-cooked in an autoclave at
two different temperatures (140 and 145 ◦C) for 30 min. The autoclaved
starch paste was allowed to cool to room temperature and
then stored at 4 ◦C for various periods of time (24, 48 and 72 h).
The autoclaving-cooling cycle was repeated three times, then oven
dried at 45 ◦C and grounded, sifted through 212 m sieve.
In prior studies autoclaving process was made at 133 ◦C as the
highest temperature. In this study, higher autoclaving temperatures
than this such as 140 ◦C and 145 ◦C were applied.
tarch.
Autoclaving temperature and storing time had no effects on TO and TP.