1.0 g lyophilized algae biomass was milled and extracted with
20 mL n-hexane with the assistance of pulse sonication at 400 w
for 20 min in an ice-bath, and the resulted supernatant was recovered
after centrifugation at 4250 g for 10 min. The above extraction
process was repeated until the resulted supernatant became colorless.
All resulted supernatants were collected and evaporated in a
rotary evaporator to remove the solvent. The residual crude algal
oil was purified by silica gel column chromatography. n-hexane
was used as the mobile phase solvent. The eluent was collected
in a pre-weighed tube and evaporated in a rotary evaporator. The
residual algal oil was desiccated to a constant weight under nitrogen
flow. The resulted weight was defined as the total algal oil (W1)
in the algal biomass.