, the diameter of 20 Nanofiber was measured randomly which was mean diameter. The conventional way of measuring electrospun fiber diameter is to analyze the micrograph manually. The manual method normally consists of the following
steps: determining the length of a pixel in the image (setting the scale), measuring the number of pixels between two edges of a fiber perpendicular to its axis, converting the number of pixels to nm using the scale and recording the result. Typically 20 diameters are measured through a histogram of fiber.