A variety of techniques can be used to determine and estimate the presence of such phytochemical compounds, including saponins. Chromatography techniques are the most useful and popular tools used for this purpose. Chromatography is an analytical technique dealing with the separation of closely related compounds from a mixture [20]. Various chromatography methods like Thin layer chromatography (TLC) and High Pressure Liquid Chromatography (HPLC) are commonly used.