Thirty shrimp (8-9g) from rach tank in the previous experiment were randonry sampled and stocked in 12 x 500-L fiberglass tanks with four repilcate tanks per treatment. V.parahaemolyticus were added into each tank to obtain final concentration of 10 CFU/ml. each treatment group received aforementioned diets four times daily for 30 days At the end of the trial there