The ratio of Monacolin K and Citrinin in each of MFR crude extract was calculated. Concentration of citrinin is one of important factor in optimization of extraction because Citrinin is regarded as toxic compound in MFR. After comparing the result, the use of 80% and 95% v/v ethanol concentration for MFR extraction gave the highest M/C ratio (see Table 2.). In Table 2, the study was compared with previous investigation10, we found that extraction method by use of 95% and 80% v/v ethanol, 2 hours extraction and under temperature of 60oC is better than previous condition that use of 30% v/v ethanol, 1 hour extraction and temperature of 65oC. The longer extraction time gave the better yield. Although by use of 80% and 95% v/v ethanol concentration gave the similar of M/C ratio, but the highest yield of Monacolin K was found by use of 95% v/v ethanol solution (MFR95) (See Fig.2). It might happened because of the level polarity from ethanol 80% v/v is higher than 95% v/v and Monacolin K is more soluble in ethanolic solution therefore the higher of ethanol concentration increased the yield of Monacolin K.
The ratio of Monacolin K and Citrinin in each of MFR crude extract was calculated. Concentration of citrinin is one of important factor in optimization of extraction because Citrinin is regarded as toxic compound in MFR. After comparing the result, the use of 80% and 95% v/v ethanol concentration for MFR extraction gave the highest M/C ratio (see Table 2.). In Table 2, the study was compared with previous investigation10, we found that extraction method by use of 95% and 80% v/v ethanol, 2 hours extraction and under temperature of 60oC is better than previous condition that use of 30% v/v ethanol, 1 hour extraction and temperature of 65oC. The longer extraction time gave the better yield. Although by use of 80% and 95% v/v ethanol concentration gave the similar of M/C ratio, but the highest yield of Monacolin K was found by use of 95% v/v ethanol solution (MFR95) (See Fig.2). It might happened because of the level polarity from ethanol 80% v/v is higher than 95% v/v and Monacolin K is more soluble in ethanolic solution therefore the higher of ethanol concentration increased the yield of Monacolin K.
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