This study reported a high titer gluconic acid fermentation using a typical lignocellulosic biomass corn stover as feedstock after it was pretreated using dry dilute sulfuric acid method (DDAP) (Zhang et al., 2011 and He et al., 2014) and enzymatically hydrolyzed into fermentable sugars. An industrial strain A. niger SIIM M276 was used as fermenting strain using the freshly prepared corn stover hydrolysate without inhibitor removal. High titer gluconic acid with the high overall yield was obtained and the obtained sodium gluconate was satisfied when it was used as cement additive. This study provided the first high titer gluconic acid production case from lignocellulosic feedstock with potential of industrial applications.