3.1. Nucleotide Sequence Distances
A pairwise percentage distance matrix of 10 Muscidae fly species is shown in Table 4. Because only 1 individual COI sequence was obtained for H. occulta, intraspecific variation was not estimated for this species. Interspecific distance was the lowest between O. chalcogaster and O. leucostoma (6.3%) and the highest between Musca domestica and Phaonia aureola (15.3%). Intraspecific distances were 0.3% or less.