2.3. Effects of different types and concentrations of auxin in combinations
with optimal cytokinin concentration on shoot proliferation
Based on the results obtained fromthe preceding experiment, shoottip
explants (1.0–1.5 cmlength) were inoculated on 20 ml MSmedium
supplemented with 5 μM mT alone and in combination with different
concentrations (0.5, 1.0 and 2.0 μM) of NAA or indole-3-butyric acid
(IBA). Full strength MS medium without any PGR was used as the control.
Cultures were grown under constant light (30 μmol m−2 s−1 photosynthetic
photon flux) at 25±2 °C. A replicate of 20 jars (2 shoot-tips
per jar) was prepared for each of the concentrations. After 12 weeks of
culture, the number of adventitious shoots produced per explant, number
of adventitious shoots 5–10 mm long, number of adventitious
shoots greater than 10 mm in length, length of longest shoot and
shoot fresh weight were recorded.