Photopatch test in human volunteers
The assay was performed according to Neumann
et al. (2000) in a limited group of 5 healthy females,
aged 27-62. Test concentrations were selected
according to the EpiDerm phototoxicity test results,
i.e. for each bergamot oil the highest non-phototoxic/
non-cytotoxic concentration of the samples diluted in
water and sesame oil was employed. The test samples
were applied in occlusion (Finn Chamber, USA),
using saturated filter paper discs (diameter of 10
mm), on the lower back in two areas on both sides of
the spine. After 1 h exposure and patch test removal