sample was dispersed in 1.0 ml of 0.2 M phosphate buffer
(pH 6.9). About 20 mg of pancreatic α amylase dissolved
in 50 ml of the same buffer and 0.5 ml of it was added to
the sample suspension and incubated at 37 °C for 2 h.
About 2 ml of 3–5 dinitrosalicylic acid (10% acqueous
solution) was added and the mixture was heated for 5 min
in a water bath. After cooling, the solution was made up to
25 ml with distilled water and filtered prior to measurement
of absorbance at 550 nm. A blank was run simultaneously