การสกัดสารประกอบและกิจกรรมการต้านเชื้อราโดยดิสก์กระดาษวิธีห้าสายพันธุ์ได้รับการคัดเลือก( จากวัฒนธรรมคู่กิจกรรมต้านเชื้อราวิธีการสกัดสารเคมีและการตรวจสอบศักยภาพต้านเชื้อราและพวกเขาBacillus methylotrophicus CNU082012 tequilensis CNU082075, Paenibacillus jamilae CNU082099 , Burkholderia cepacia CNU082111 aeruginosa CNU082142 Compound extraction and antifungal activity by paper disk
method
Five isolates were selected (minimum one isolate from one
antagonistic genus) by dual culture antifungal activity method
for chemical extraction and antifungal potentiality check and
they were Bacillus methylotrophicus CNU082012, B.
tequilensis CNU082075, Paenibacillus jamilae CNU082099,
Burkholderia cepacia CNU082111 and Pseudomonas
aeruginosa CNU082142. Compound of CNU082012 and
CNU082075 from Hexane, chloroform and ethyl acetate
soluble portions did not show any activity against B. cinerea.
The ethyl acetate soluble portion of CNU082099 and
CNU082142 showed weak antagonistic activity (Fig. 4). Two
Bacillus isolates CNU082012 and CNU082075 showed activity
when tested with compound separated by butanol. Extracts
from bacterial cells were dissolved in methanol and checked
their antagonistic activity against B. cinerea. Two bacillus
isolates (B. methylotrophicus CNU082012 and B.
amyloquifaciens CNU082075) and the Burkholderia cepacia
CNU082111 were strongly active as antifungal agent against B.
cinerea. The antagonistic activity of B. cepacia CNU082111
was strongest against Botrytis cinerea by paper disk method
(Fig. 4). The inhibition zone was >55 mm (data not shown).
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