For this purpose, the MPNDPCR
estimation of the number of cells was compared with
the direct count. For quantitative analysis, 107 cells in 50 ll
(2 · 108 cells ml)1), a
s determined by direct cell counts,
were added to the initial tubes of five replicate dilution
series. The cell concentration in the original suspension as
estimated by MPN-DPCR was 1Æ58 · 108 cells ml)1 (95%
confidence limits ¼ 4Æ80 · 107 to 5Æ23 · 108).