This study showed that methanolic extract of Azadirachta
indica leaves was an effective antibacterial and antisecretory
agent againstVibrio cholerae, the causative agent of dreadful disease
cholera. Additionally, it was also found that neem extract
had antihemorrhagic activity. The active extract found in this
work may be employed for the treatment of the patient infected
with Vibrio cholerae belonged to serotypes O1, O139 and non-
O1, non-O139. Emergence of multiply drug-resistant Vibrio
cholerae is a serious clinical problem in the treatment and containment
of the disease. However, the active leaves extract in this
work had bactericidal activity against different strains of multidrug-
resistant Vibrio cholerae. This could be suggested that the
active Azadirachta indica leaves extract found in our work may
be used as potential source to develop novel antimicrobial compound
and antisecretory drug useful to treat cholera and diarrheal
patients. The results obtained in this study give some scientific
support to the indigenous uses of neem in India employed for
the treatment of diarrhea and cholera. Further efforts should be
directed at investigating the principle in this extract which has
these antibacterial, antisecretory and antihemorrhagic activities
against Vibrio cholerae.