Additionally, to obtain a chromatographic profile of the extract, High Performance Liquid Chromatography (HPLC) analyses were performed on a Shimadzu LC10ADvp system equipped with a Supelco LC 18 column (Supelcosil™ RP-18, 2504.6 mm; 5lm) and the elutes were monitored by diode array detector SPDM 10 A Shimadzu at 250 nm.