For each of the two prebiotics, each of four 10mL
aliquots of PY broth containing 1% (w/v) FOS or IMO
were inoculated separately with one of the four bacterial
strains (from WC agar) and incubated anaerobically at
37 C overnight. An aliquot of 0.5mL of each overnight
culture was then transferred to 59.5mL PY broth
containing the appropriate prebiotic (1% w/v) and was
further incubated under the same conditions. After 16 h
of incubation, 10mL of one probiotic strain and 10mL
of one pathogen was used to inoculate 80 mL of prereduced
PY broth containing 1% (w/v) of the appropriate
prebiotic to give four different tests in total (two
synbiotics each with two pathogens). In addition, 10mL
of each pathogen was used to inoculate 90 mL of sterile
PY broth containing 1% (w/v) of the appropriate
prebiotic (two prebiotics each with two pathogens)