Table 2 shows the detection limit of the seven PCR
methods. In this study, the ITS1- and the SSU-rRNAPCR
were the most sensitive methods that could detect
L. siamensis at the concentration as low as 2.3 pg/μl or
0.05 parasites/μl. In contrast, five other PCR methods
could amplify when higher concentrations of L. siamensis
DNA were applied. The PCR method amplifying the
ITS1 region of L. siamensis showed the amplicon of 348 bp,
while amplifying L. donovani DNA generated the amplicon
of 319 bp.