Advances in biomedical science are leading to upsurge synthesis of nanodelivery systems for drug delivery. The
systems were characterized by controlled, targeted and sustained drug delivery ability. Humans are the target of
these systems, hence, animals whose systems resembles humans were used to predict outcome.
Thus, increasing costs in money and time, plus ethical concerns over animal usage. However, with consideration
and planning in experimental conditions, in vitro pharmacological studies of the nanodelivery can mimic the in vivo
system. This can function as a simple method to investigate the effect of such materials without endangering
animals especially at screening phase.
Keywords: In vitro, Animal studies, Nanodelivery system, Toxicity and bio distribution