Immunocytochemical localization of (A) pPRLR-MPFlag and (B) hPRLR-Flag (C) pPRLR-MPFlag and LPG-A and (D) hPRLR-Flag and LPG-A proteins expressed in transiently transfected CHO-K1 cells. No background fluorescence in untransfected CHO-K1 cells was observed (not shown). Flag epitope expression is in red (Cy3), LPG-A protein expression is in green (Alexa Fluor 488) and 4′,6-diamidino-2-phenylindole (DAPI) stained nuclei are blue. Immunoreactive proteins were detected as described in the materials and methods. Some highly concentrated circular regions of cytoplasmic PRLR staining are indicated with arrows. Scale bar = 5.00 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)