Preparation
of
decimal
dilutions
of
VJs,
Ins
and
KLBs
was
in
Ringer's
solution
(SigmaeAldrich, Milan, Italy).
The
cell
suspensions
were
used
to
estimate
the
following
microbial
groups:
total
mesophilic
count
(TMC)
on
plate
count
agar
(PCA),
incubated
aerobically
at
30 C
for 72 h;
Enterobacteriaceae
on
double-layered
violet
red
bile
glucose
agar
(VRBGA),
incubated
aerobically
at 37 C
for 24 h;
pseudomonads
on
Pseudomonas
agar
base
(PAB)
supplemented
with
10 mg/mL
cetrimide
fucidin,
incubated
aerobically
at
20 C
for
48 h;
rod
LAB
on
de
Man-Rogosa-Sharpe
(MRS)
agar,
acidified
to
pH 5.4
with
lactic acid
(5 mol/L)
and
incubated
anaerobically
at
30 C
for 48 h;
coccus
LAB
on
M17
agar,
incubated
anaerobically
at
30 C
for 48 h;
yeasts
on
dichloran
rose
Bengal
chloramphenicol
(DRBC)
agar,
incubated
aerobically
at
25 C
for
48 h.
All
media
and
supplements
were
purchased
from
Oxoid
(Milan, Italy).
Count
plates
were
carried
out
in
duplicate
for
each
independent
production.