Sequential injection chromatography (SIC) is a young, ten years old, separation technique. It was
proposed with the benefits of reagent-saving, rapid analysis, system miniaturization and simplicity. SIC with UV
detection has proven to be efficient mostly for pharmaceutical analysis. In the current study, a stand-alone
multi-wavelength fluorescence (FL) detector was coupled to an SIC system. The hyphenation was exploited
for developing an SIC-FL method for the separation and quantification of amiloride (AML) and furosemide
(FSM) in human urine and tablet formulation