The concept of simultaneous saccharification and fermentation
for ethanol production using WBFB could be successfully applied
without adding enzymes from outside because WBFB contains high
amounts of carbon, nitrogen and other substances including saccharifying
enzymes from malt. The ethanol producing yeast strains
i.e. S. cerevisiae and C. krusie and some bacterial species were found
to exist in WBFB. This whole study proved WBFB as a potential
ethanol producing source which did not require the addition of any
strain, enzyme or nutrient. With finding the proper stock time of
WBFB, a proper method to encourage yeast cells and depress other
bacterial cells during ethanol production should be developed in
future studies in order to get the utmost productivity