Southern blot analyses of genomic DNA from leaves of transgenic
rice plants produced by particle bombardment of immature embryos.
Genomic DNA (5 mg) was restricted with SacI. The 480 bp GNA coding
sequence amplified by PCR was used as the probe. Lane 1, one gene copy
equivalent of plasmid pRSSGNA restricted with SacI. Lanes 2–6, DNA from
plants transformed with pRSSGNA; lanes 7–11 and 13, DNA from plants
transformed with pUbiGNA; lane 12, DNA from control rice plant.