and pump (LC-10AT VP, “Shimadzu”). Separations were performed on an “Adsorbosil”
column with NH grups (150 mm × 4.6 mm). The mobile phase of 75% acetonitrile in
2
double distilled water was used. Three analytical samples of sugars (n = 3) were
measured for each treatment.
Plants were harvested on 22
and 30 day after sowing and oven-dried at
nd th
+105°C for 24 h to determine the dry weight. Leaf area of cucumber plants was
measured by “WinDias” leaf area meter (“Delta-T Devices Ltd”, UK). Plant height was
measured up to the top of cucumber transplants.
Organogenesis stages of cucumber plants were identified according to metho-
dology of F. М. Kuperman /Куперман и др., 1982/ and biometric and physiological
indices were determined in five replicates ( n = 5) at the end of cultivation of transplants
(30 days after sowing). Ripe fruits were harvested from eight ( n = 8) plants three times
per week.
The levels of significance for differences between various indices were analysed
using one-way Anova (Anova for MS Excel, version 3.43). The results were expressed as
mean values and their standard errors (SE) using MS Excel software. Significant
differences from reference treatment are denoted by an asterisk (*) at P ≤ 0.05