In the present study, the freeze-dried microparticles ALGL and AHQL had mean diameters of 114.51 μm and 112.50 μm, respectively. The structural change caused by the freeze-drying process is often referred to cause an increase in pore size (Nakagawa, Iwamoto, Nakajima, Shonob, & Satohb, 2004), allowing a fast and complete rehydration (Fellows, 2006).
For the treatment ALGL, the size difference may be related to hydration capacity of polysaccharides. Chemical side groups such as COO- and SO3 in polysaccharides can interact with water molecules via hydrogen bridges (Boudou et al., 2010).