2.4. Collection of testes and epididymal sperm
Pairs of testes and epididymides were collected from the
three male CryoXeno pigs slaughtered between 2.1 and
2.5 years of age. Three pieces, excised from different portions
of the left testis of each pig, were fixed in Bouin solution
and embedded in paraffin for histologic examination.
Testicular pieces were also collected from four conventional
boars of the same age (Landrace Large
White Duroc crossbreed, born at the National Institute of
Livestock and Grassland Science). Sperm were collected
from epididymides of CryoXeno pigs and frozen using the
methods reported by Kikuchi, et al. [14].