In our opinion, RP-HPLC methods are more suitable for qualitative studies of c-oryzanol components and for a small number of samples. The screening of a large number of samples requires a fast and reliable sample preparation and the possibility of automated and replicated HPLC injections. In NP-HPLC, the huge amount of apolar waxes, TAG and FFA are eluted before the analytes of interest thus eliminating the need of long column rinsing and tedious preliminary cleaning-up of samples. Therefore, our aims are: (1) to explore the potential application of NP-HPLC in separating coryzanol components, (2) to develop and validate a simple and informative NP-HPLC method for the routine quantification of coryzanol.