After collecting the washed biomass in the biomass buffer tank, the pretreated EFB was transferred into the hydrolysis tanks using a screw conveyer. For this study, the SHF process was used for producing ethanol from EFB. Each hydrolysis batch included two pretreatment batches and an enzyme cocktail that was supplied to each pretreatment batch. The first supplied biomass batch was hydrolyzed for almost 20 h and the second supplied biomass batch was hydrolyzed for 14 h. The pretreated biomass was simply kept overnight in the hydrolysis tanks for producing glucose. The reasons for minimizing the hydrolysis time are the inhibition effect of glucose and cellobiose accumulation in the hydrolysis tank. The inhibitor substantially reduces the enzyme velocity at low substrate concentrations [26]. The hydrolyzate was transferred into the fermentation tanks by pump, and subsequently, 5% S. cerevisiae was added for converting the glucose to ethanol. Table 4 lists the results of the bioethanol production process. Every fermentation batch produced 40.6 ± 0.3 g/L bioethanol, which included the ethanol from enzyme and seed mash from 11.7 ± 0.3% biomass concentration. Bioethanol of concentration 36.9 ± 0.3 g/L was produced from only biomass. The theoretical amount of produced bioethanol was 44.1 ± 0.4 g/L and ethanol conversion rate was 83.6%. Although the conversion rate was high, the ethanol concentration was not suitable for economical distillation and dehydration. A process for increasing the ethanol concentration should be developed to reduce the production cost.