2.1. Cell Culture. Human hepatocellular carcinoma cell lines
Mahlavu, PLC, and SMMC7721 were kindly given by Dr Li-
JianHui of the Institute of Biochemistry and Cell Biology of
ChineseAcademy of Sciences (Shanghai,China) and cultured
in Dulbecco’s modified Eagle’s medium (DMEM, Hyclone)
containing 10% fetal bovine serum (Hyclone) and penicillin/
streptomycin (100 units/mL and 100