Murrah buffaloes (Chaudhary et al. 2011), and Surti
buffaloes (Singh et al. 2011). Clone libraries, however,
require a large numbers of clones to be analyzed, thus
making it a cumbersome approach. The lack of consistency
in the procedures of nucleic acid isolation, PCR
conditions, and OTU determination makes quantification
and statistical comparisons among clone libraries
difficult (Zoetendal et al. 2004). Hence, clone libraries
are generally constructed in parallel to DNA fingerprinting
techniques that allowed well-informed decisions
on the number of clones to be sequenced.