Ajwain from the Apiaceae family exhibits several biological effects including analgesic, anti-inflammatory, anxiolytic and antispasmodic activities. Its seed has been widely consumed as a food flavoring agent and spice. Moreover, the seeds have several therapeutic effects and have been used for treatments of indigestion, flatulence, colic, diarrhea, and dyspepsia. Total essential oil of ajwain has large amounts of carvacrol or thymol. These phenolic compounds are expressed to be either bactericidal or bacteriostatic agents depending on the concentration