Retention of vitamin C in homogenized raw fruits and vegetables stored under routine conditions prior
to analysis was investigated. Raw collard greens (Brassica oleracea var. viridis), clementines (Citrus
clementina hort. ex Tanaka), and potatoes (Solanum tuberosum) were chosen, being representative of
foods to be sampled in USDA’s National Food and Nutrient Analysis Program (NFNAP), and having
different expected stability of ascorbic acid (AA). Samples were homogenized in liquid nitrogen, assayed
immediately, then stored at 60 8C and analyzed at time points up to 49 weeks. Vitamin C (as total AA
after reduction of dehydroascorbic acid) was analyzed using a validated method with quantitation by
HPLC/ultraviolet detection. An orange juice control sample was included in each run. Vitamin C
concentrations were stable in clementines and the orange juice, but decreased in collards and potatoes
[16.8 and 10.9 mg/100 g (14.7% and 30.4%), respectively, after 49 weeks]. Significant losses had occurred
after 12 weeks. These results suggest similar matrices must receive careful attention to sample handling
protocols before analysis or AA values may not reflect the concentration in the food as consumed. The
control sample was critical to allowing assessment of storage effects independent of analytical
variability. Fruits and vegetables for the NFNAP will be analyzed without storage until a practical
stabilization protocol is validated.
Retention of vitamin C in homogenized raw fruits and vegetables stored under routine conditions priorto analysis was investigated. Raw collard greens (Brassica oleracea var. viridis), clementines (Citrusclementina hort. ex Tanaka), and potatoes (Solanum tuberosum) were chosen, being representative offoods to be sampled in USDA’s National Food and Nutrient Analysis Program (NFNAP), and havingdifferent expected stability of ascorbic acid (AA). Samples were homogenized in liquid nitrogen, assayedimmediately, then stored at 60 8C and analyzed at time points up to 49 weeks. Vitamin C (as total AAafter reduction of dehydroascorbic acid) was analyzed using a validated method with quantitation byHPLC/ultraviolet detection. An orange juice control sample was included in each run. Vitamin Cconcentrations were stable in clementines and the orange juice, but decreased in collards and potatoes[16.8 and 10.9 mg/100 g (14.7% and 30.4%), respectively, after 49 weeks]. Significant losses had occurredafter 12 weeks. These results suggest similar matrices must receive careful attention to sample handlingprotocols before analysis or AA values may not reflect the concentration in the food as consumed. Thecontrol sample was critical to allowing assessment of storage effects independent of analyticalvariability. Fruits and vegetables for the NFNAP will be analyzed without storage until a practicalstabilization protocol is validated.
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