In addition, the leaf essential oil (1%) with low fat milk at 250 IU/mL nisin severely decreased the cell count numbers, resulting in no cell growth formation of L. monocytogenes ATCC 19116 at 1% leaf essential oil in combination with 500 IU/mL of nisin. Also 62.5 and 125 IU/mL treatments of nisin with 1% leaf essential oil exhibited strong antilisterial effect in low fat milk as compared to control group. No growth of L. monocytogenes ATCC 19116 was observed at further combined concentrations of oil and nisin in low milk sample