The means of the F2-mycelial running rate for Shiitake and Lingzhi is described in Figure 4. Compared to the means of the F2-mycelial running rate from the control substrate (100% corn seeds), the result showed that F2-mycelial growth of Shiitake and Lingzhi from alternative substrates (F1-mycelial grew on 60% corn seeds and 40% tuber) was not significantly different (P>0.05). The time required for completion of mycelium running of F2-mycelial Shiitake was 28 days, which is in accordance with the results of a study by Yanamaka [8], whereas F2-mycelial Lingzhi ranged from 25 days to 30 days.