Methionine and Lys were determined by acid hydrolysis. Calcium was determined ,and P was measured by converting phosphates into phosphorus molybdenum blue pigment and measured at 700 nm . Salt was determined by AOAC International method . Saponin content was measured by HPLC . Tannin content,expressed as tannic acid equivalents, was measured based on the Folin-Ciocalteu method . The total polysaccharides were measured by the vitriol-anthracene ketone method, with glucose without H2O as the standard control .